OrangerV1, Main, Exploration, bibRecord, 001932

Production and evaluation of transgenic sweet orange (Citrus sinensis Osbeck) containing bivalent antibacterial peptide genes (Shiva A and Cecropin B) via a novel Agrobacterium-mediated transformation of mature axillary buds

Identifieur interne : 001932 ( Main/Exploration ); précédent : 001931; suivant : 001933

Production and evaluation of transgenic sweet orange (Citrus sinensis Osbeck) containing bivalent antibacterial peptide genes (Shiva A and Cecropin B) via a novel Agrobacterium-mediated transformation of mature axillary buds

Auteurs : YONGRUI HE [République populaire de Chine] ; SHANCHUN CHEN [République populaire de Chine] ; AIHONG PENG [République populaire de Chine] ; XIUPING ZOU [République populaire de Chine] ; LANZHENG XU [République populaire de Chine] ; TIANGANG LEI [République populaire de Chine] ; XIAOFENG LIU [République populaire de Chine] ; LIXIAO YAO [République populaire de Chine]

Source :

Scientia horticulturae [ 0304-4238 ] ; 2011.

RBID : Pascal:11-0170386

Descripteurs français

Pascal (Inist)
- Peptide, Gène, Transformation génétique, Transfection, Maturité, Bourgeon axillaire, Bactériose, Horticulture, Organisme génétiquement modifié, Citrus sinensis, Agrobacterium, Agrume, Antibactérien, Chancre plante, Résistance aux maladies.
Wicri :
- topic : Horticulture, Organisme génétiquement modifié, Agrume.

English descriptors

KwdEn :
- Agrobacterium, Antibacterial agent, Axillary bud, Bacteriosis, Citrus fruit, Citrus sinensis, Disease resistance, Gene, Genetic transformation, Genetically modified organism, Horticulture, Maturity, Peptides, Plant cankers, Transfection.

Abstract

Transgenic plants of Jincheng orange (Citrus sinensis Osbeck) and Newhall navel orange (C. sinensis Osbeck) containing antibacterial peptide genes Shiva A and Cecropin B were successfully obtained by a novel Agrobacterium tumefaciens-mediated transformation of the mature axillary buds. PCR and Southern blot analysis of the transgenic plants verified that the Shiva A and Cecropin B genes were integrated into the citrus genome. The transgenic plants began to blossom and bear fruit in the 2nd year after grafting on trifoliate orange (Poncirus trifoliata Raf) rootstock in greenhouse. Water-soluble extracts from transgenic citrus leaves exhibit in vitro suppressive effects on Xanthomonas axonopodis pv. citri, suggesting that the expressed products of Shiva A and Cecropin B in citrus retain their native antibacterial activities. Artificial inoculation in greenhouse and open field further indicates significantly increased resistance of transgenic plants to X. axonopodis pv. citri when compared with non-transgenic lines. No significant difference was found in the content of total soluble solids, total acidity, reduced sugar content and other fruit characteristics between transgenic and non-transgenic plants. In this present study, 11 transgenic lines were obtained from 40 transgenic lines, showing enhanced resistance to citrus canker disease.

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000186
to stream PascalFrancis, to step Curation: 000814
to stream PascalFrancis, to step Checkpoint: 000153
to stream Main, to step Merge: 001962
to stream Main, to step Curation: 001932

Le document en format XML

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<sZ>6 aut.</sZ>
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</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>National Citrus Engineering Research Center</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Center for Citrus Variety Improvement</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Center for Citrus Variety Improvement</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aihong Peng" sort="Aihong Peng" uniqKey="Aihong Peng" last="Aihong Peng">AIHONG PENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research Institute, Chinese Academy of Agricultural Sciences</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Chongqing 400712</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>National Citrus Engineering Research Center</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>National Citrus Engineering Research Center</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Center for Citrus Variety Improvement</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>National Center for Citrus Variety Improvement</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xiuping Zou" sort="Xiuping Zou" uniqKey="Xiuping Zou" last="Xiuping Zou">XIUPING ZOU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research Institute, Chinese Academy of Agricultural Sciences</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Chongqing 400712</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>National Citrus Engineering Research Center</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>National Citrus Engineering Research Center</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Center for Citrus Variety Improvement</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>National Center for Citrus Variety Improvement</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lanzheng Xu" sort="Lanzheng Xu" uniqKey="Lanzheng Xu" last="Lanzheng Xu">LANZHENG XU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research Institute, Chinese Academy of Agricultural Sciences</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>Chongqing 400712</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>National Citrus Engineering Research Center</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Citrus Engineering Research Center</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Center for Citrus Variety Improvement</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Center for Citrus Variety Improvement</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tiangang Lei" sort="Tiangang Lei" uniqKey="Tiangang Lei" last="Tiangang Lei">TIANGANG LEI</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research Institute, Chinese Academy of Agricultural Sciences</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>Chongqing 400712</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>National Citrus Engineering Research Center</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Citrus Engineering Research Center</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Center for Citrus Variety Improvement</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Center for Citrus Variety Improvement</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xiaofeng Liu" sort="Xiaofeng Liu" uniqKey="Xiaofeng Liu" last="Xiaofeng Liu">XIAOFENG LIU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research Institute, Chinese Academy of Agricultural Sciences</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>Chongqing 400712</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>National Citrus Engineering Research Center</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Citrus Engineering Research Center</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Center for Citrus Variety Improvement</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Center for Citrus Variety Improvement</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lixiao Yao" sort="Lixiao Yao" uniqKey="Lixiao Yao" last="Lixiao Yao">LIXIAO YAO</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research Institute, Chinese Academy of Agricultural Sciences</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
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<sZ>7 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>Chongqing 400712</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>National Citrus Engineering Research Center</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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<sZ>8 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>National Citrus Engineering Research Center</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>National Center for Citrus Variety Improvement</s1>
<s2>Chongqing 400712</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>National Center for Citrus Variety Improvement</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Scientia horticulturae</title>
<title level="j" type="abbreviated">Sci. hortic.</title>
<idno type="ISSN">0304-4238</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Scientia horticulturae</title>
<title level="j" type="abbreviated">Sci. hortic.</title>
<idno type="ISSN">0304-4238</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium</term>
<term>Antibacterial agent</term>
<term>Axillary bud</term>
<term>Bacteriosis</term>
<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Disease resistance</term>
<term>Gene</term>
<term>Genetic transformation</term>
<term>Genetically modified organism</term>
<term>Horticulture</term>
<term>Maturity</term>
<term>Peptides</term>
<term>Plant cankers</term>
<term>Transfection</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Peptide</term>
<term>Gène</term>
<term>Transformation génétique</term>
<term>Transfection</term>
<term>Maturité</term>
<term>Bourgeon axillaire</term>
<term>Bactériose</term>
<term>Horticulture</term>
<term>Organisme génétiquement modifié</term>
<term>Citrus sinensis</term>
<term>Agrobacterium</term>
<term>Agrume</term>
<term>Antibactérien</term>
<term>Chancre plante</term>
<term>Résistance aux maladies</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Horticulture</term>
<term>Organisme génétiquement modifié</term>
<term>Agrume</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Transgenic plants of Jincheng orange (Citrus sinensis Osbeck) and Newhall navel orange (C. sinensis Osbeck) containing antibacterial peptide genes Shiva A and Cecropin B were successfully obtained by a novel Agrobacterium tumefaciens-mediated transformation of the mature axillary buds. PCR and Southern blot analysis of the transgenic plants verified that the Shiva A and Cecropin B genes were integrated into the citrus genome. The transgenic plants began to blossom and bear fruit in the 2nd year after grafting on trifoliate orange (Poncirus trifoliata Raf) rootstock in greenhouse. Water-soluble extracts from transgenic citrus leaves exhibit in vitro suppressive effects on Xanthomonas axonopodis pv. citri, suggesting that the expressed products of Shiva A and Cecropin B in citrus retain their native antibacterial activities. Artificial inoculation in greenhouse and open field further indicates significantly increased resistance of transgenic plants to X. axonopodis pv. citri when compared with non-transgenic lines. No significant difference was found in the content of total soluble solids, total acidity, reduced sugar content and other fruit characteristics between transgenic and non-transgenic plants. In this present study, 11 transgenic lines were obtained from 40 transgenic lines, showing enhanced resistance to citrus canker disease.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Yongrui He" sort="Yongrui He" uniqKey="Yongrui He" last="Yongrui He">YONGRUI HE</name>
</noRegion>
<name sortKey="Aihong Peng" sort="Aihong Peng" uniqKey="Aihong Peng" last="Aihong Peng">AIHONG PENG</name>
<name sortKey="Aihong Peng" sort="Aihong Peng" uniqKey="Aihong Peng" last="Aihong Peng">AIHONG PENG</name>
<name sortKey="Aihong Peng" sort="Aihong Peng" uniqKey="Aihong Peng" last="Aihong Peng">AIHONG PENG</name>
<name sortKey="Lanzheng Xu" sort="Lanzheng Xu" uniqKey="Lanzheng Xu" last="Lanzheng Xu">LANZHENG XU</name>
<name sortKey="Lanzheng Xu" sort="Lanzheng Xu" uniqKey="Lanzheng Xu" last="Lanzheng Xu">LANZHENG XU</name>
<name sortKey="Lanzheng Xu" sort="Lanzheng Xu" uniqKey="Lanzheng Xu" last="Lanzheng Xu">LANZHENG XU</name>
<name sortKey="Lixiao Yao" sort="Lixiao Yao" uniqKey="Lixiao Yao" last="Lixiao Yao">LIXIAO YAO</name>
<name sortKey="Lixiao Yao" sort="Lixiao Yao" uniqKey="Lixiao Yao" last="Lixiao Yao">LIXIAO YAO</name>
<name sortKey="Lixiao Yao" sort="Lixiao Yao" uniqKey="Lixiao Yao" last="Lixiao Yao">LIXIAO YAO</name>
<name sortKey="Shanchun Chen" sort="Shanchun Chen" uniqKey="Shanchun Chen" last="Shanchun Chen">SHANCHUN CHEN</name>
<name sortKey="Shanchun Chen" sort="Shanchun Chen" uniqKey="Shanchun Chen" last="Shanchun Chen">SHANCHUN CHEN</name>
<name sortKey="Shanchun Chen" sort="Shanchun Chen" uniqKey="Shanchun Chen" last="Shanchun Chen">SHANCHUN CHEN</name>
<name sortKey="Tiangang Lei" sort="Tiangang Lei" uniqKey="Tiangang Lei" last="Tiangang Lei">TIANGANG LEI</name>
<name sortKey="Tiangang Lei" sort="Tiangang Lei" uniqKey="Tiangang Lei" last="Tiangang Lei">TIANGANG LEI</name>
<name sortKey="Tiangang Lei" sort="Tiangang Lei" uniqKey="Tiangang Lei" last="Tiangang Lei">TIANGANG LEI</name>
<name sortKey="Xiaofeng Liu" sort="Xiaofeng Liu" uniqKey="Xiaofeng Liu" last="Xiaofeng Liu">XIAOFENG LIU</name>
<name sortKey="Xiaofeng Liu" sort="Xiaofeng Liu" uniqKey="Xiaofeng Liu" last="Xiaofeng Liu">XIAOFENG LIU</name>
<name sortKey="Xiaofeng Liu" sort="Xiaofeng Liu" uniqKey="Xiaofeng Liu" last="Xiaofeng Liu">XIAOFENG LIU</name>
<name sortKey="Xiuping Zou" sort="Xiuping Zou" uniqKey="Xiuping Zou" last="Xiuping Zou">XIUPING ZOU</name>
<name sortKey="Xiuping Zou" sort="Xiuping Zou" uniqKey="Xiuping Zou" last="Xiuping Zou">XIUPING ZOU</name>
<name sortKey="Xiuping Zou" sort="Xiuping Zou" uniqKey="Xiuping Zou" last="Xiuping Zou">XIUPING ZOU</name>
<name sortKey="Yongrui He" sort="Yongrui He" uniqKey="Yongrui He" last="Yongrui He">YONGRUI HE</name>
<name sortKey="Yongrui He" sort="Yongrui He" uniqKey="Yongrui He" last="Yongrui He">YONGRUI HE</name>
</country>
</tree>
</affiliations>
</record>

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Production and evaluation of transgenic sweet orange (Citrus sinensis Osbeck) containing bivalent antibacterial peptide genes (Shiva A and Cecropin B) via a novel Agrobacterium-mediated transformation of mature axillary buds

Production and evaluation of transgenic sweet orange (Citrus sinensis Osbeck) containing bivalent antibacterial peptide genes (Shiva A and Cecropin B) via a novel Agrobacterium-mediated transformation of mature axillary buds

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